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Fluorescent Reagents for Live Cell Imaging and Their Introduction ... Fluorescent Reagents for Live Cell Imaging and Their Introduction into Cells. Ilan Davis and Richard M. Parton. This information panel was adapted from ... 2.
Normal saline -- 2007 (19): pdb.rec11154 -- Cold Spring Harbor ... Normal saline. recipe 125 mM NaCl. recipe 2.5 mM KCl. recipe · caution 2 mM CaCl2. recipe · caution 1.3 mM MgSO4. recipe 25 mM NaHCO3. 1.25 mM NaH2PO4 ... 3.
LB agar + carbenicillin plates -- 2008 (9): pdb.rec11374 -- Cold ... recipe · caution Carbenicillin (50 mg/mL). To prepare LB agar plates with 50 µg/mL carbenicillin, combine 32 g of LB agar with 1 L of H2O. Stir and ... 4.
Dideoxy-mediated Sequencing Reactions Using the Klenow Fragment of ... This protocol was adapted from Molecular Cloning, 3rd edition, by Joseph Sambrook and David W. ... Klenow fragment of E. coli DNA polymerase I (5 units/µl) ... 5.
Preparation and Microinjection of x-Rhodamine-Labeled Vimentin ... Add 40:1 molar excess of the labeling agent 5- (and 6-) carboxy-x-rhodamine succimidyl ester, dimethylformamide to 10% (v/v), and NaCl to a final ... 6.
Pipette solution for K+ current recordings -- 2008 (7): pdb ... Pipette solution for K+ current recordings. 10 mM HEPES. recipe 20 mM KCl. 80 mM potassium glutamate. recipe Before each experiment, add Mg-ATP from a 0.5 M ... 7.
Design and Cloning of an shRNA into a Lentiviral Silencing Vector ... pENTR/U6 has a ccdB toxic gene and must therefore be propagated in tolerant strains ... Design an shRNA and clone into pENTR/U6. Digest pENTR/U6 with BsaI, ... 8.
Microinjection buffer B -- 2007 (1): pdb.rec10795 -- Cold Spring ... Microinjection buffer B. 100 mM PIPES. 1 mM MgCl2. recipe 1 mM EGTA (pH 6.8-7.0). Recipe. EGTA (ethylene glycol-bis[β-aminoethyl ether]-N,N,N' ... 9.
Preparation of a Prepacked IMAC Column -- Haneskog 2006 (1): pdb ... Preparation of a Prepacked IMAC Column. Lars Haneskog. This protocol was adapted from "Immobilized Metal-Ion Affinity Chromatography," contributed by Lars ... 10.
Loading and Running DNA Sequencing Gels -- Sambrook and Russell ... The sets of nested DNA fragments generated by DNA sequencing methods are resolved by .... Turn off the power supply and disconnect the sequencing apparatus. ... |
