1.
Electroporation
-Early on Monday feed the cells 4 hours before electroporation. The procedure will take an hour to half a day to complete. (A dense plate of R1s will ...
2.
Raising Mice
It is easiest to sex newborn mice if the genital region of the mouse is fully ..... Mouse care and husbandry. In: "Mouse Genetics and Transgenics: A ...
3.
Online Resources
Lee Silver's Mouse Genetics, published online by the Jackson Laboratory; The Coat Colors of Mice by Willys K. Silvers, published online by the Jackson ...
4.
IACUC Protocol Preparation for Transgenic and Knockout Mice
Writing an IACUC protocol for new transgenic or knockout mice. The Trangenesis and Targeting Service has an approved protocol for the production of ...
5.
Frozen Embryo Transfer
Frozen Embryo Transfer (FET) is the thawing of cryopreserved embryos and their transfer into recipient mothers to generate live born offspring. ...
6.
Southerns
Once you've accumulated some long exposures to be sure of the entire plate, get the positives out of the 96-well freeze ASAP. After thawing them you'll ...
7.
Electroporation
Electroporation Of Targeting/Transfection Vector. We generally follow the Wurst and Joyner transfection protocol (chapter 2 from Gene Targeting: a practical ...
8.
Generation of mice harboring a Sox5 conditional null allele
File Format: PDF/Adobe Acrobat - View as HTML Prx1Cre newborn littermates. The left pictures show that the ribs and vertebrae of the .... cages of newborn mice and cultured as described (Lefeb- ...
9.
Regulatory Issues for Investigators Using Transgenic and Knockout Mice
To add new transgenics or chimeras, investigators with currently approved ... "Biosafety Level 1 is suitable for work involving well-characterized agents ...
10.
Gene Targeting
We provide detailed information about the design of gene targeting vectors. Genetic Background and Gene Targeting In the past, most ES cell lines used in ...
