1.
ChipperDB: DGAP Experiment
Hybridization was performed with U74av2 chips by the Dana-Farber Cancer Institute microarray core facility. PGC-1a in myocytes, Mouse, myoblast/myotube ...
2.
Real-time quantitative RT-PCR (Taqman)
File Format: PDF/Adobe Acrobat - View as HTML if the 5′ gene-specific Taqman primer is located greater than about 2-3 kb ... PCR protocol (multiplex reaction). Before the PCR can be performed on the ...
3.
Real-time quantitative RT-PCR (Taqman)
File Format: PDF/Adobe Acrobat - View as HTML all samples to be investigated. Reagent. Volume (for 1 sample). Taqman universal PCR mix. 10.0 ul. Target. Forward primer 0.2 ul gene. Reverse primer 0.2 ul ...
4.
IV. Assay for Conversion of U- C-Glucose, H-Glycerol or C-pyruvate
File Format: PDF/Adobe Acrobat - View as HTML as radio-labeled substrates, into the glyceride moiety of triglyceride. Thus these assays can be used to estimate rates of esterification of fatty acyl CoA with ... and is then converted into the glycerid
5.
ChipperDB: DGAP Experiment
Total RNA was extracted using Trizol method and used for cRNA synthesis. Hybridization was performed with U74av2 chips by Dr. Vamsi Mootha. ...
6.
V. RNA Isolation From Primary Fat Cells using Trizol from ...
File Format: PDF/Adobe Acrobat - View as HTML 1. Prepare primary fat cells according to the Primary Fat Cell protocol. 2. Remove the entire amount of buffer from the fat cells and add Trizol at 1ml per ...
7.
1) ISOLATION and PRIMARY CULTURE of BROWN FAT PREADIPOCYTES ...
File Format: PDF/Adobe Acrobat - View as HTML Stock Oil Red O: 0.5g Oil Red O (Sigma, catalog # 0-0625) in 100 ml isopropanol. 1. Rinse plates with PBS. 2. Fix cells by covering with PBS + 10% ...
8.
T16 - Introduction to Bioinformatics A. J. Butte, MD 11/8/03 1:00 ...
File Format: PDF/Adobe Acrobat - View as HTML Nov 8, 2003 ... Data types in bioinformatics Link. • Parallels between medical and bio- informatics Link. • Developing diagnostic tests Link ...
9.
V. RNA Isolation From Primary Fat Cells using Trizol from ...
File Format: PDF/Adobe Acrobat - View as HTML V. RNA Isolation From Primary Fat Cells using Trizol from Invitrogen. Principal Investator: Michael P. Czech. This protocol is used by the UMass Genomics ...
10.
I. ISOLATION OF MOUSE OR RAT PRIMARY FAT CELLS Principal ...
File Format: PDF/Adobe Acrobat - View as HTML The epidydimal fat pad is isolated from the mice(or rat) and placed in a 100 x 20 mm tissue culture dish with a small amount of KRH+2.5% BSA buffer to keep ...
