1.
TBS (Tris-buffered saline)
TBS (Tris-buffered saline) - 1L. 25 mM Tris, 150 mM NaCl, 2 mM KCl, pH 7.4. 1X TBS, 10X TBS ... Tris base. 30 g. Tris base. 800 ml. ddH2O. 800 ml. ddH2O ...
2.
Transfer Buffer ( for Western blotting )
To make 1X Transfer Buffer from 10X: Mix 100 ml of 10X Transfer Buffer, 100 ml of methanol and 800 ml of ddH2O per liter ** ...
3.
Calculate Polyacrylamide gel recipes for SDS-PAGE
Calculate Polyacrylamide gel recipes for SDS-PAGE. Just enter the number of gels and the ... Acrylamide. ml, 1.5M Tris pH 8.8. µl, 10% SDS. µl, 10% APS ...
4.
Dynabead Immunoprecipitation
1) Wash 100 µl of Dynabeads twice with 500 µl of 1X IP Buffer using the MPC (use the same species-specific beads as are used for the actual ...
5.
Dynabead Immunoprecipitation
1) Wash 100µl of species-specific Dynabeads® (depending on the origin of your precipitating antibody) three times with 500 µl of 1X PBS by repeated ...
6.
Cytographica.com - Lab Tools
SDS-PAGE Recipe Calculators. Calculate Recipes for SDS-PAGE gels by entering the desired percent polyacrylamide and total final volume ...
7.
Cytographica - Animations
Request this animation ... Protein structure information of EGFR obtained from: The Protein Data Bank ... Binding of EGF causes a conformational change ...
8.
RIPA Buffer
RIPA (RadioImmunoPrecipitation Assay) Buffer - 100 ml. 50 mM Tris-Cl pH 7.4, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS. Reagents needed: ...
9.
TAE (Tris-acetate-EDTA)
TAE (Tris-acetate-EDTA) - 1L. 40 mM Tris-acetate, 1 mM EDTA ... 1) Dissolve Tris base in 800 ml of ddH2O by stirring. 2) Add glacial acetic acid and EDTA ...
10.
Cold Water Fish Skin Gelatin Blocking Buffer
0.5% of Cold Water Fish Skin Gelatin (Teleostean Gelatin) in 1X phosphate buffered saline - pH 7.4 with 0.1% Triton X-100 and 0.1% casein. Reagents needed: ...
