1.
PROTEIN ASSAY
The advantage of this assay over the Lowry assay from which it is derived, is the presence of SDS in reagent A. This allows one to perform protein assays in ...
2.
CROSSLINKING OF PROTEINS
A shorter reaction time would be preferable, to minimize fortuitous crosslinking of protein. Add 5 µl 1 M Tris-HCL pH 8.5 (an excess) to stop the ...
3.
MEDIA D4 DULBECCO’S MINIMAL EAGLE’S MEDIA (DMEM) 13.40 g DMEM ...
File Format: PDF/Adobe Acrobat - View as HTML DULBECCO’S MINIMAL EAGLE’S MEDIA (DMEM). 13.40 g. DMEM (Gibco 430-2100). 3.70 g. Sodium bicarbonate. 2.38 g. Hepes base (Sigma H2275) ...
4.
BLACK LIPID BILAYER APPARATUS
because this lipid is less sensitive than cholesterol and need more proteins (but it also gives less artifact than with cholesterol). Record events on chart ...
5.
PROTEIN ASSAY
The advantage of this assay over the Lowry assay from which it is derived, is the presence of SDS in reagent A. This allows one to perform protein assays in ...
6.
DOUBLE IMMUNODIFFUSION
DOUBLE IMMUNODIFFUSION. REFERENCE: Ouchterlony, (1959). MATERIALS: 0.08% barbitone buffer: 12.0 g sodium 5'5 diethyl barbiturate ...
7.
MURAMIC ACID ASSAY
(Purpose to assay muramic acid, a component of peptidoglycan). STOCK SOLUTIONS: ... Read absorbance at 560 nm. Standard curve: 0 - 20 µg muramic acid. ...
8.
RsaL
Barbara H. Iglewski, University of Rochester; Luciano Passador, University of Gene characterisation publicised as part of the Pseudomonas Community ...
9.
MURAMIC ACID ASSAY
(Purpose to assay muramic acid, a component of peptidoglycan). STOCK SOLUTIONS: ... Read absorbance at 560 nm. Standard curve: 0 - 20 µg muramic acid. ...
10.
METHYLATION OF FATTY ACIDS (KROPINSKI METHOD)
Add internal standard (C15 fatty acid) to give a final concentration of 400 nmoles/100ml (usually 10mg of C15 in 100ml of reagent. ...
